Abstract: Interferon is a kind of cytokines with broad biological activity， it has a wide range of antitumor effect. Interferon family includes three types, type Ⅰ， type Ⅱ and type Ⅲ. Studies have shown that type Ⅰ interferon especially INF-α2b which combined with 5-FUbased chemotherapy can play a certain role in biliary tract cancer，and INF-α combined with HELF chemotherapy regimen is a nice regimen for advcanced gallbladder carcinoma.In addition to better immunomodulatory effects， type Ⅱ interferon can inhibit biliary tumor cell and promote apoptosis.When type Ⅱ interferon combined with CaMantagonist or Mifeptistone or Chinese medicine can be synergistic. Type Ⅲ interferons currently uses only for the treatment of viral diseases， but basic researches clearly demonstrate its antitumor effect， it is worth further study. This article reveiws the progression of intereron in treating biliary tract cancer.