Abstract: Gamma delta（γδ） T cells are minor subset of T lymphocytes（2％-5％ of peripheral blood T lymphocytes） that express Vγ9Vδ2 T cell receptors， mainly distributed in the mucosaassociated lymphoid tissue, are nonrestricted cells of major histocompatibility complex（MHC）， can kill various tumor cells. Many data demonstrate that γδ T cells are involved in the first line of immune defense system. In the future， adoptive γδ T cellsbased cell immunotherapy will become a new strategy for cancer immunotherapy. In this review， we provide an overview of its antitumor mechanism and clinical application.