Abstract:

Objective To investigate the expression levels of retinoic acid receptor 3 （RARRES3） and migration and invasion enhancement factor 1 （MIEN1） in non-small cell lung cancer （NSCLC） tissues and their relationship with clinical pathological features and prognosis. Methods In this study， 86 cases of NSCLC tissues and 72 cases of adjacent tissues were collected from our hospital. The expression of RARRES3 and MIEN1 in cancer tissues and adjacent tissues were detected by immunohistochemical SP method， and the expression level was divided into low expression and high expression level according to the two-grade score. The relationship between the expression level of two protein and clinical pathological parameters （gender， age， pathological type， tumor size， differentiation type， TNM stage， T stage， smoking history and lymph node metastasis） were analyzed. We further investigated the correlation between both proteins.
Results The positive particles of RARRES3 and MIEN1 were located in the cytoplasm. The expression of RARRES3 in NSCLC tissues was 29.07％ （25/86）， lower than 55.56％ （40/72） of para-carcinoma tissues （P＜0.05）， while the expression of MIEN1 in NSCLC tissues was 62.79％ （54/86）， higher than 31.94％（23/72） of para-carcinoma tissues （P＜0.05）. The expression of RARRES3 and MIEN1 protein in NSCLC was related to TNM stage and lymph node metastasis. Moreover， RARRES3 was also associated with tumor size， and MIEN1 protein was also associated with differentiation type （P＜0.05）. RARRES3 protein expression were negatively correlated with MIEN1 （r=-0.411，P=0.000）. The AUC， sensitivity and specificity of RARRES3 and MIEN1 in NSCLC were 0.825 and 0.779， 85.1％ and 75.4％， 79.2％ and 83.3％， respectively. The median overall survival （OS） was 32.5 months in the RARRES3 high expression group， which was higher than 16.0 months in the low expression group， and the median OS was 15.5 months in MIEN1 high expression group， which was lower than 28.0 months in the low expression group （P＜0.05）. Conclusion In NSCLC tissues， RARRES3 was lowly expressed and MIEN1 was highly expressed. Both proteins were related to TNM staging， lymph node metastasis and prognosis. It may be related to the occurrence and development of NSCLC.