Abstract: Objective To determine the expression of basic leucine zipper nuclear factor 1（BLZF1）in gastric cancer and analyze the relationship of its expression with clinical features and prognosis. Methods The expression level of BLZF1 was detected by tissue microarray technology and immunohistochemistry in 156 cases of gastric cancer and paired adjacent normal tissues. The relationship between the expression of BLZF1 and clinical parameters were analyzed as well as overall survival（OS）. Results The positive rate of BLZF1 in gastric cancer tissues was 52.56％（82／156）， lower than 95.51％（149／156）of paracarcinoma tissues（P＜0.05）. The expression of BLZF1 was related with T stage， TNM stage and differentiation degree（P＜0.05）， but unrelated with age， sex， tumor size and N stage（P＞0.05）. The median OS for the patients with negative expression of BLZF1 was 29.0 months， shorter than 42.5 months of patients with positive expression of BLZF1（P＜0.05）. Multivariate analysis revealed that T stage， tumor size， TNM stage and BLZF1 expression were independent prognostic factors for gastric cancer patients. Conclusion BLZF1 is down-regulated in gastric cancer and may be related to tumor genesis and development. Loss of BLZF1 expression is associated with the poor prognosis of gastric cancer.