Abstract: Objective To investigate the clinical significance of Latexin（LXN） in esophageal squamous cell carcinoma（ESCC）. Methods In this study， 183 cases of ESCC tissues and para-carcinoma tissues were evaluated for LXN expression by tissue microarray and immunohistochemistry. The relationship between the expression of LXN and clinical parameters（tumor size， T staging, N stagings, differentiation and TNM staging） and prognosis were analyzed. Results The positive rate of LXN expression was 48.6% in ESCC tissues， lower than 100.0% in para-carcinoma tissues. A significant correlation was observed between the expression of LXN and tumor size， T staging, N staging， tumor differentiation and TNM staging. Patients with LXN positive expression had longer overall survival than those without LXN expression. Conclusion LXN was down-regulated with the development and progression of ESCC and loss of LXN expression is associated with the poor prognosis of ESCC.