Abstract: Objective To investigate the role of Fn14 with JAK2／STAT3 pathway in esophageal squamous cell carcinoma（ESCC）， and the expression of JAK2／STAT3 molecules were detected and the correlation of Fn14 with JAK／STAT was discussed. Methods Fn14，p-JAK2 and p-STAT3 expressions in tumor tissues were assessed using immunohistochemical（IHC） methods in 118 patients with resected ESCC， and the correlation of Fn14 expression with JAK2／STAT3 expression was analyzed. Results The expression of Fn14 was mainly located on cell membrane and the expression of p-JAK2， p-STAT3 were mainly located in cytoplasm and cell nucleus. The positive expression rates of Fn14， p-JAK2 and p-STAT3 protein in ESCC were 51.7％（61／118）， 50.9％（60／118） and 57.6％（68／118）， which were higher than 4.2％（5／118） of corresponding adjacent non-cancerous tissue with statistical difference（P＜0.05）. There were significant differences for Fn14， p-JAK2， p-STAT3 expressions in pathological grades and TNM stages groups（P＜0.05）， while there were no significant differences for Fn14， p-JAK2， p-STAT3 in sex and age （P＞0.05）. The correlation of Fn14 with p-JAK2， p-STAT3 were obvious in pathological grades and TNM stages in ESCC（P＜0.05）. Conclusion The Fn14， p-JAK2， p-STAT3 proteins were highly expressed in ESCC， which suggest that Fn14， p-JAK2， p-STAT3 would be likely to be associated with the development of ESCC.