Abstract: Objective To investigate the expression of GRP78 and KIAA1324 proteins in gastric cancer and their correlation with clinicopathological parameters and prognosis. MethodsImmunohistochemical technique was used to detect the expression of GRP78 and KIAA1324 proteins in 90 cases of gastric cancer tissues. The relationship between the expression of GRP78 and KIAA1324 proteins and clinicopathological parameters was analyzed. The survival rate was calculated by Kaplan-Meier method. The survival differences were analyzed by Log-rank test. Results The positive rates of GRP78 and KIAA1324 were 78.89％（71/90） and 35.56％（32/90）， respectively. The expression of GRP78 and KIAA1324 was correlated with lymph node metastasis and TNM stage （P＜0.05）. The expression of GRP78 was negatively related to expression of KIAA1324（r=-0.469，P＜0.05）.The median survival time of patients with positive expression of GRP78 was 49.78 months（95％CI：44.09~55.46），which was lower than 62.21 months（95%CI: 53.29~71.14） of patients with negative expression of GRP78，with significant difference（P＜0.05）. The median survival time of patients with positive expression of KIAA1324 was 60.66 months（95％CI：53.63~67.69）， which was higher than 47.85 months（95%CI: 41.46~54.23） of patients with negative expression of KIAA1324， with significant difference（P＜0.05）. Conclusion GRP78 and KIAA1324 play important roles in generation and development of tumor， and they may serve as promising targets for therapy of gastric cancer.