Abstract: Objective To investigate the relationship between the expression of B7-H3 and the level of tumor-associated macrophages（TAMs） infiltration in hepatocellular carcinoma（HCC） tissues. MethodsThe expression level of B7-H3 was detected by immunohistochemistry and quantitative Real-time PCR in 116 HCC tissues. Immunohistochemistry also was used to detect CD68 expression. Then the relationship between B7-H3， CD68 expression and clinicopathologic features was analyzed. Results Among the 116 cancer tissues， the high expression rate of B7-H3 was 79.3％（92／116）； and patients with the low， middle， high level of TAMs infiltration were 21， 49 and 46 cases. The expression of B7-H3 was related to TNM stage， vascular invasion and microsatellite lesions formation（P＜0.05）； The level of TAMs infiltration was related to TNM stage， vascular invasion， lymph node metastases and microsatellite lesions formation（P＜0.05）. The mRNA level of B7-H3 was significantly correlated with the level of TAMs infiltration （r=0.473，P＜0.001）. ConclusionThe expression level of B7-H3 is positively correlated with the level of TAMs infiltration in HCC tissues, and they may contribute to the oncogenesis and progression of HCC.