Abstract: Objective To investigate the effect of microRNA-133b （miR-133b） on glioblastoma （GBM） cell and to analyze its possible molecular mechanism. Methods Reverse transcription-polymerase chain reaction（RT-PCR） was used to detect the expression of miR-133b in 20 human GBM samples and normal human glial cells HBE. Transwell migration and invasion assays were used to evaluate the effects of miR-133b on cell migration and invasion. Western blotting and a luciferase reporter assay were used to identify the target genes of miR-133b. Results Compared with the HBE， miR-133b was significantly increased in GBM tissues （1.0±0.17 vs. 2.42±0.69, P＜0.05）； In Transwell invasion and migration experiments， compared to group transfected with NC， the cells transfected with miR-133b mimic could inhibit the migration and invasion （P＜0.05）； In Western blotting， compared to cells that transfected with NC， the cells transfected with miR-133b mimic had a lower expression of MMP-14 protein （P＜0.05）. Furthermore， MMP-14 was identified as a direct target gene of the miR-133b by luciferase reporter gene analysis. Conclusion miR-133b can inhibit the migration and invasion of GBM by direct targeting MMP-14, and it can be used as a candidate target for diagnosis and treatment of GBM.