Abstract:

Objective To investigate the expression of acid-sensing ion channel 3（ASIC-3） and pain behavior in mice skin cancer pain after intrathecal amiloride.
MethodsBalb／c mice were randomly divided into 4 groups： Normal group （group N）， Cancer pain group （group C）， Saline group （group S） and Amiloride group （group A）. Each group was 10 mice. Except group N， the skin cancer pain model was set up through inoculating subcutaneously tumor cells into paw of mice. Group N and group C were not intervented by drugs. By intrathecal injection， the mice of group S were given 10 μl saline and mice of group A were given 15 μg/μl amiloride solution. Thermal withdrawal latency （TWL） of mice in each group on 0，30，60，120，180 minutes after temperature stimulation were detected respectively. At last， expression of ASIC-3 of spinal dorsal horn in mice was detected by Western blotting method. Results The TWL of each mice has no difference before inoculating（P＞0.05）. The TWL of group C，group S and group A was decreased from（9.66±0.54）s，（9.96±0.39）s，（10.15±0.40）s to （4.32±0.25）s，（4.16±0.33）s，（4.26±0.24）s after 2 weeks inoculation（P＜0.05）. On 60，120，180 minutes after injection， the TWL in group A was（6.03±0.23）s，（7.09±0.24）s，（6.49±0.19）s，which was higher than those of group C and group S（P＜0.05）. The relative expression of ASIC-3 in spinal cord of group A was 1.88±0.11， which was lower than that of group C（2.23±0.32） and group S（2.43±0.24）， and the difference was statistically significant （P＜0.05）. Conclusion Amiloride has the effect of inhibiting the ASIC-3 expression of the spinal dorsal horn in skin cancer pain mice， and taking part in the feeling of pain.