Abstract: Objective To investigate the regulation of microRNA-92a（miR-92a）on the proliferation and apoptosis of nasopharyngeal carcinoma cell line HONE1 by targeting PTEN／Akt signaling pathway. Methods The miR-92a inhibitor（miR-92a group）and the negative control（NC group）were transfected into HONE1 cells by Lipofectamine liposome，and the HONE1 cells without transfection were used as control group. QPCR was used to detect the expression of miR-92a in different cell groups after transfection. The cell proliferation rate and apoptotic rate were detected by MTT method and flow cytometry，respectively. The expressions of PTEN and p-Akt in PTEN／Akt signaling pathway were measured by Western blotting. Results 48 h after transfection，the expression of miR-92a in miR-92a group was significantly lower than that in control group and NC group（P＜0.05），while the difference between control group and NC group were not statistically significant（P＞0.05）. The proliferation rates of miR-92a group after transfection of 24，48，72 and 96 h were（84.51±2.74）％,（77.21±3.55）％,（62.07±3.57）％ and （49.25±4.15）％，the data of 72 h and 96 h were lower than control group and NC group（P＜0.05）. The apoptotic rate of miR92a group at 48 h after transfection was（46.12±1.79）％，higher than（6.99±0.72）％ of control group and（8.42±0.81）％ of NC group，and the difference was statistically significant（P＜0.05）. The expression levels of PTEN and p-Akt were 0.61±0.12 and 0.37±0.09 in miR-92a group，0.41±0.11 and 0.73±0.14 in control group, and 0.39±0.08 and 0.68±0.07 in NC group at 48 h after transfection. Compared with control group and NC group，elevated level of PTEN expression and decreased level of p-Akt expression were observed in miR-92a group（P＜0.05）. Conclusion Inhibiting the expression of miR-92a can inhibit the proliferation of nasopharyngeal carcinoma cells and promote its apoptosis，which may be achieved by negative regulation of PTEN／Akt signaling pathway.