Abstract:

Objective To explore the role of microRNA-375（miR-375）on the invasion and migration of nasopharyngeal carcinoma cells and its targeting regulation of Janus activated kinase 2／signal transducer and activator of transcription 3（JAK2／STAT3）signaling pathway. Methods Nasopharyngeal carcinoma CNE-1 cells were cultured and transfected with mi-375 mimics（miR-375 group）and miR-375 negative control（NC group）via Lipofectamine 2000 liposomes. CNE-1 cells without transfection were used as control group. The expression of miR-375 in each group at 48 h after transfection was detected by real-time quantitative PCR（QPCR）. The migration and invasion ability of the cells were detected by scratch test and Transwell test，respectively. Western blotting was used to detect the changes of JAK2 and STAT3 proteins in JAK2／STAT3 signaling pathway.
Results The results of QPCR showed that the expression of miR-375 in miR-375 group was 10.74±1.21，higher than 1.09±0.14 of NC group and 1.12±0.17 of control group（P＜0.05）. The migration rate of miR-375 group was（18.22±1.78）％，lower than（43.67±2.60）％ of control group and（49.70±1.31）％ of NC group（P＜0.05）. The number of transmembrane cells in miR375 group was 45.76±4.34，also lower than 144.98±3.65 of control group and 126.23±6.32 of NC group，and the difference was statistically significant（P＜0.05）. The expression of JAK2 and STAT3 protein were 0.31±0.06 and 0.27±0.05 in miR-375 group，0.54±0.05 and 0.41±0.06 in control group, and 0.50±0.06 and 0.43±0.07 in NC group. The expression of JAK2 and STAT3 in miR-375 group were lower than those in the other two groups（P＜0.05）. There was no significant difference between NC group and control group（P＞0.05）. Conclusion Up-regulation of miR-375 expression can inhibit the invasion and migration of CNE-1 cells. It may be related to the inhibition of JAK2／STAT3 signaling pathway.