Abstract: Objective To identify and excise sentinel lymph nodes in endoscopic inguinal lymphadenectomy of vulvar cancer，and look for pathway of lymphatic transferation of vulvar cancer. Methods Three patients bearing vulvar squamous-cell cancer were enrolled in this study. Methylthioninium chloride injection was subcutaneously and intracutaneously injected at upper outer side of tumors before endoscopic inguinal lymphadenectomy. During the process of lymphadenectomy，blue stained lymph nodes and lymph-vessels were found and their locations were confirmed. Results All blue stained sentinel lymph nodes and lymph-vessels were identified and excised，and the sentinel lymph nodes were those located near tuberculum pubicum at upper inner side of superficial epigastric vein. Conclusion Identification and excision of sentinel lymph node is easy under endoscopic inguinal lymphadenectomy of vulvar cancer. Lymph nodes near tuberculum pubicum are significant sentinel lymph nodes of vulvar cancer.