KCNJ15在肺腺癌组织中的表达及其对细胞增殖迁移的影响#br#

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临床肿瘤学杂志 ›› 2018, Vol. 23 ›› Issue (8): 685-689.

KCNJ15在肺腺癌组织中的表达及其对细胞增殖迁移的影响#br#
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湖北省十堰市太和医院肿瘤科武汉华中科技大学同济医学院附属同济医院肿瘤科

出版日期:2018-08-31 发布日期:2018-09-07

Expression of KCNJ15 in lung adenocarcinoma tissues and its effect on cell proliferation and migration

Department of Oncology， Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology

Online:2018-08-31 Published:2018-09-07

摘要/Abstract

摘要： 目的探讨内向整流钾通道J亚家族成员15（KCNJ15）在肺腺癌组织中的表达及其对肺腺癌A549细胞增殖和迁移的影响。
方法收集本院2006年7月至2015年10月经手术切除的肺腺癌组织117例和癌旁组织50例，采用实时定量PCR法检测以上组织中KCNJ15 mRNA水平，分析肺腺癌组织KCNJ15 mRNA水平与临床病理特征（性别、年龄、肿瘤大小、临床分期、肿瘤位置、淋巴结转移和病理分级）的关系，根据随访资料分析不同KCNJ15 mRNA水平的预后情况。构建过表达KCNJ15的载体并转染A549细胞，采用CCK8法和Transwell试验分析KCNJ15基因在肺腺癌细胞增殖和迁移中的功能。
结果与癌旁组织相比，肺腺癌组织中KCNJ15 mRNA水平降低（P＜005）；KCNJ15表达与肺腺癌患者的肿瘤大小和临床分期有关（P＜005），与年龄、病理分级、性别、淋巴结转移和肿瘤位置无关（P＞005）；KCNJ15高表达的肺腺癌患者的总生存期长于低表达者；获得性功能实验结果表明，KCNJ15基因过表达能够抑制肺腺癌细胞的增殖和迁移能力。
结论KCNJ15基因在肺腺癌中的表达明显降低，其表达与肺腺癌患者的肿瘤大小和临床分期有关，在肺腺癌患者生存中是一个重要的有利预后标志物，可作为潜在的肺腺癌临床治疗新靶标。

关键词: 肺腺癌, 内向整流钾通道J亚家族成员15, 临床意义, 增殖迁移

Abstract: ObjectiveTo investigate the expression of potassium inwardlyrectifying channel subfamily J member 15 （KCNJ15） in lung adenocarcinoma tissues and its effect on proliferation and migration of lung adenocarcinoma A549 cells.
MethodsA total of 117 lung cancer tissues and 50 paracancerous tissues were collected from July 2006 to October 2015 in our hospital. The mRNA levels of KCNJ15 in the above tissues were detected by realtime quantitative PCR， and the relationship between the mRNA level of KCNJ15 in the lung adenocarcinoma tissues and the clinicopathological parameters （sex， age， tumor size， clinical stage， tumor location， lymph node metastasis and pathological grade） was analyzed. The prognosis of different KCNJ15 mRNA levels was analyzed according to the followup data. The overexpression vector of KCNJ15 was constructed and transfected into A549 cells. The function of KCNJ15 gene on proliferation and migration of A549 cells was analyzed by CCK8 and Transwell assay.
ResultsThe mRNA level of KCNJ15 gene in lung adenocarcinoma tissues was significantly lower than that of paracancerous tissues （P＜005）. The expression of KCNJ15 gene was related to the size and clinical stage of the lung adenocarcinoma （P＜005）， but not related to age， pathological grade， sex， lymph node metastasis and tumor location （P＞005）. The overall survival of the lung adenocarcinoma patients with high expression of KCNJ15 was longer than patients with low expression. The results of the acquired functional experiment showed that the overexpression of KCNJ15 gene could inhibit the proliferation and migration of lung adenocarcinoma cells.
ConclusionThe expression of KCNJ15 gene in lung adenocarcinoma is obviously decreased. Its expression is related to the size and clinical stage of lung adenocarcinoma. It is an important prognostic marker in the survival of the patients with lung adenocarcinoma， and can be used as a potential new target for the clinical treatment of lung adenocarcinoma.

Key words: Lung adenocarcinoma, Potassium inwardlyrectifying channel subfamily J member 15, Clinical significance, Proliferation and migration

刘东伯, 杨丽, 周洋媚, 陈春丽. KCNJ15在肺腺癌组织中的表达及其对细胞增殖迁移的影响#br#

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[J]. 临床肿瘤学杂志, 2018, 23(8): 685-689.

LIU Dongbo, YANG Li, ZHOU Yangmei, CHEN Chunli. Expression of KCNJ15 in lung adenocarcinoma tissues and its effect on cell proliferation and migration[J]. Chinese Clinical Oncology, 2018, 23(8): 685-689.

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