宫颈癌组织中微小RNA155水平及其对细胞增殖和侵袭的影响#br#

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临床肿瘤学杂志 ›› 2018, Vol. 23 ›› Issue (8): 690-695.

宫颈癌组织中微小RNA155水平及其对细胞增殖和侵袭的影响#br#
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湖北医药学院附属随州医院肿瘤科

湖北随州随州市妇幼保健院妇产科

出版日期:2018-08-31 发布日期:2018-09-07

Expression of microRNA155 in cervical cancer tissues and its effect on cell proliferation and invasion of cervical cancer cells

Department of Obstetrics and Gynecology， Suizhou Maternal and Child HealthCare Hospital

Online:2018-08-31 Published:2018-09-07

摘要/Abstract

摘要： 目的探讨微小RNA155（miR155）在宫颈癌组织中的表达及其对宫颈癌细胞增殖和侵袭的影响。
方法收集本院2014年5月至2016年12月经手术切除的宫颈癌组织76例、正常宫颈组织63例及宫颈上皮内瘤样病变（CIN）组织84例（CINⅠ级22例、CIN Ⅱ级29例和CIN Ⅲ级33例），采用实时定量PCR（QPCR）检测以上组织中的miR155水平，分析miR155水平与宫颈癌临床病理特征（年龄、FIGO分期、肿瘤大小、组织学分级、病理类型、血管侵犯和淋巴结转移）的关系；向宫颈癌HeLa细胞分别转染miR155抑制物（转染组）和阴性对照（对照组），采用QPCR检测转染48 h后各组的miR155水平，分别采用MTT法和Transwell侵袭实验检测增殖率和穿膜细胞数目。
结果QPCR检测发现宫颈癌组织中的miR155水平为4270±1901，高于正常宫颈组织和CIN组织（P＜005）；miR155水平与宫颈癌患者的年龄、组织学分级和病理类型均无关（P＞005），但与FIGO分期、肿瘤大小、血管侵犯和淋巴结转移有关（P＜005）。转染miR155抑制物48 h后，转染组HeLa细胞的miR155水平为0265±0034，低于对照组的1027±0158，差异有统计学意义（P＜005）；与对照组相比，转染组的增殖率和穿膜细胞数均降低，差异有统计学意义（P＜005）。
结论miR155在宫颈癌组织中呈高表达，且与临床分期、血管侵犯和淋巴结转移有关，参与宫颈癌的发生发展。降低miR155水平可抑制增殖和侵袭过程，在宫颈癌的防治中有一定价值。

关键词: 宫颈癌, 微小RNA155, 临床意义, 增殖, 侵袭

Abstract: ObjectiveTo explore the expression of microRNA155 （miR155） in cervical cancer tissues and its effect on proliferation and invasion of cervical cancer cells.
MethodsWe collected 76 cases of cervical cancer tissues， 63 cases of normal cervical tissue and 84 cases of cervical intraepithelial neoplasia tissues （22 cases of CIN Ⅰ， 29 cases of CIN Ⅱ and 33 cases of CIN Ⅲ） from May 2014 to December 2016 in our hospital. The miR155 levels in the above tissues were detected by realtime quantitative PCR （QPCR）， and the relationship between the miR155 level and the clinicopathological parameters of cervical cancer （age， FIGO staging， tumor size， histological grade， pathological type， vascular invasion and lymph node metastasis） was analyzed. HeLa cells were transfected with miR155 inhibitor （transfection group） and negative control （control group）， and the miR155 level of each group after transfection of 48 h was detected by QPCR. The proliferative rate and the cell number of penetrating membrane were detected by MTT and Transwell invasiveness test， respectively.
ResultsQPCR detection showed that the miR155 level in cervical cancer tissues was 4270±1901， higher than that in normal cervical tissues and CIN tissues （P＜005）. The level of miR155 was not related to age， histological grade and pathological type of cervical cancer （P＞005）， but it was related to FIGO staging， tumor size， vascular invasion and lymph node metastasis （P＜005）. The miR155 level of cervical cancer cells transfected with miR155 inhibitor at 48 h was 0265±0034， lower than 1027±0158 of the control group （P＜005）. Compared with the control group， the proliferative rate and the number of penetrating membrane in the transfection group were lower （P＜005）.
ConclusionMiR155 is highly expressed in cervical cancer tissues， and is associated with clinical stage， vascular invasion and lymph node metastasis. MiR155 is involved in the development of cervical cancer. Downregulating the level of miR155 can inhibit the proliferation and invasion process， and has a certain value in the prevention and treatment of cervical cancer.

Key words: Cervical cancer, MicroRNA155, Clinical significance, Proliferation, Invasion

陈玉容, 谢辉, 唐慧, 吴莉, 储音越, 郑洁. 宫颈癌组织中微小RNA155水平及其对细胞增殖和侵袭的影响#br#

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[J]. 临床肿瘤学杂志, 2018, 23(8): 690-695.

CHEN Yurong, XIE Hui, TANG Hui, WU Li, CHU Yinyue, ZHENG Jie.. Expression of microRNA155 in cervical cancer tissues and its effect on cell proliferation and invasion of cervical cancer cells[J]. Chinese Clinical Oncology, 2018, 23(8): 690-695.

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