Abstract: Objective To investigate the inhibitory effect of tumstatin lentiviral vector （Lenti-Tum） on the growth of transplanted hepatic carcinoma in nude mice and explore its possible application in the gene therapy of human hepatocarcinoma. Methods The subcutaneous hepatic carcinoma SMCC-7721 xenograft was successfully established in nude mice. Peri-tumoral and intra-tumoral injection of 100μl PBS （PBS group）， 5×109 TU of green fluorescent protein lentiviral vector （Lenti-GFP group） and 5×109 TU Lenti-Tum （Lenti-Tum group） were carried out in nude mice bearing human hepatic carcinoma xenograft. The tumor volumes of the three groups were observed. Reverse transcriptase polymerase chain reaction and Western blotting were employed to detect the mRNA and protein levels of tumstatin， respectively. The survival times of three groups were analyzed using the Kaplan-Meier method. Results The tumor volume of Lenti-Tum group was （702.1 ± 143.7） mm3， less than （1622.2 ± 253.8） mm3 in PBS group and （1538.5 ± 284.9） mm3 in Lenti-GFP group （P＜0.05）. The inhibitory rate of Lenti-Tum group was 56.7％， higher than 5.2％ in Lenti-GFP group （P＜0.05）. Both the mRNA and protein levels of tumstatin in LentiTum group were higher than those in other two groups （P＜0.05）.The median overall survival in Lenti-Tum group was 100 days， much longer than 58 days in PBS group and 59 days in Lenti-GFP group（P＜0.05）. Conclusion The Lenti-Tum can significantly inhibit the growth of hepatic carcinoma SMCC-7721 xenograft and prolong the overall survival time of nude mice.