Abstract:

Objective To investigate and analyze the expression of miR-21 in hepatocarcinoma cell strains and the relationship with PTEN. Methods The quantitative reverse transcription-PCR were used to detect the expression of miR-122 and PTEN in hepatocarcinoma cell strains， PTEN and p-Akt expression was detected in tumor-bearing nude mice treated with miR-21 inhibitor. Results The relative expression of miR-21 was 2.97±0.79，137.66±23.60 and 511.21±1.14 in WRL-68， MHCC97-H and SMMC-7721 strains respectively， while that was 1.29±0.33， 36.90±11.84 and 6.22±0.85 respectively when miR-21 inhibitor was transfected. The relative expression of PTEN was 2.88±0.93， 0.19±0.07 and 0.62±0.06 in WRL-68， MHCC97-H and SMMC-7721 strains respectively， while that was 6.11±1.07， 2.65±0.66 and 4.32±0.84 respectively when miR-21 inhibitor was transfected. The expression of PTEN was positive and p-Akt was down regulated when tumor-bearing nude mice was treated with miR-21 inhibitor. Conclusion MiR-21 is an upstream regulation factor of PTEN， it can affect PTEN expression.