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纳米Fe3O4/C225复合物对结直肠癌LoVo细胞放射增敏作用的初步研究

孟庆红1,孙新臣2,孙苏平1,于静萍1,倪新初1,赵迪1
  

  1. 1 213003 江苏常州南京医科大学附属常州第二人民医院放疗科 210009 南京医科大学第一附属医院江苏省人民医院放疗科
  • 收稿日期:2014-03-20 修回日期:2014-04-09 出版日期:2014-07-30 发布日期:2014-07-30
  • 通讯作者: 孙新臣

A preliminary study on radiosensitization effect of nano Fe3O4/C225 complex on human colorectal cancer LoVo cells

MENG Qinghong,SUN Xinchen,SUN Suping,YU Jingping,NI Xinchu,ZHAO Di.

  

  1. Department of Radiotherapy, the Changzhou Second People’s Hospital Affiliated to Nanjing Medical University,Nanjing 210009,China
  • Received:2014-03-20 Revised:2014-04-09 Online:2014-07-30 Published:2014-07-30
  • Contact: SUN Xinchen

摘要: 目的 探讨纳米Fe3O4/C225复合物(简称复合物)对结直肠癌LoVo细胞放射增敏性的影响。方法 采用MTT法观察不同浓度复合物(0、3.125、6.25、12.5、25、50、100mg/L)作用24h后的增殖抑制率,根据实验设计分为单纯照射组、C225+照射组及复合物+照射组,采用克隆形成实验检测3组经0、2、4、6、8和10Gy X线照射后的存活分数(SF),流式细胞仪检测3组经4Gy X线照射24h后的细胞周期情况,采用Western blotting检测经4Gy X线照射24h后的表皮生长因子受体(EGFR)蛋白水平及其相关信号通路中p-P38和p-Akt蛋白水平。结果在3.125~100mg/L范围内,随复合物浓度增加,LoVo细胞的增殖抑制率升高,0、3.125、6.25、12.5、25、50、100mg/L的增殖抑制率依次为0%、(8.950±0.086)%、(14.760±0.011)%、(29.860±0.001)%、(41.750±0.017)%、(59.770±0.010)%和(78.710±0.017)%,组间差异有统计学意义(P<0.05);复合物+照射组的SF、S期细胞比例及p-Akt、EGFR蛋白水平均低于C225+照射组和单纯照射组,G0/G1期、G2/M期细胞比例及p-P38蛋白水平均高于C225+照射组和单纯照射组,以上差异均有统计学意义(P<0.05)。结论 纳米Fe3O4/C225复合物可抑制LoVo细胞增殖并具有放射增敏作用,可能与G0/G1期阻滞及EGFR相关信号通路受抑制有关。

Abstract: Objective To investigate the radiosensitivity enhancement and possible mechanism of nano Fe3O4/C225 complex on human colorectal cancer LoVo cells. Methods In vitro inhibition rates of cell proliferation at 24h after treatment with different concentrations (0, 3.125, 6.25, 12.5, 25, 50, 100 mg/mL) of nano Fe3O4/C225 complex were determined by MTT. According to the experimental protocol, the below experiments were carried out in irradiation group,C225+irradiation group and complex+irradiation group. The colony formation assay was employed to observe the surviving fraction (SF) of three groups after X-ray irradiation of 0, 2, 4, 6, 8, 10Gy. The cell cycle of three groups at 24h after X-ray irradiation of 4Gy was measured by flow cytometry. Western blotting was employed to measure the protein level of epidermal growth factor receptor (EGFR) and p-P38, p-Akt in the related of signaling pathway.
ResultsThe proliferation inhibition rate of LoVo cells gradually increased with the increasing concentration of Fe3O4/C225 complex ranging from 3.125 to 100 mg/L. The proliferation inhibition rates were 0%, (8.950± 0.086)%, (14.760±0.011)%, (29.860±0.001)%,(41.750±0.017)%, (59.770±0.010)% and (78.710±0.017)% at the concentrations of 0, 3.125, 6.25, 12.5, 25, 50, 100mg/mL with statistically significant differences (P<0.05).Compared with irradiation group and C225+irradiation group, there were lower SF, percentage of S cells and protein levels of p-Akt,EGFR and higher percentages of G0/G1 and G2/M and protein level of p-P38 in complex+irradiation group with statistically significant differences (P<0.05). Conclusion Nano Fe3O4/C225 complex could inhibit the proliferation of LoVo cells with radiosensitizing effect possibly via the arrest at the G0/G1 phase and inhibition of EGFR signaling pathways.

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