Abstract: Objective To explore the influences of Schisandrin B （Sch B） on proliferation， apoptosis and Wnt／β-catenin signaling pathway of SKOV3 human ovarian cancer cells. Methods The SKOV3 cells were treated with different concentrations of Sch B （0， 1.0， 10.0， 20.0，50.0μmol／L）. The MTT was used to measure the proliferation inhibition rates at 24， 48，72 and 96h treated with different concentrations of Sch B. The Annexin-FITC／PI double staining was employed to detect cell apoptosis at 48h after treatment with different concentrations of Sch B. The cycle distribution at 48h after treatment with Sch B was detected by flow cytometry. The Western blotting was used to measure the nuclear β-catenin protein levels in Wnt／β-catenin signaling pathway as well as its downstream target C-myc and cyclin D1. The cytoplasm／nucleus activities of glycogen synthase kinase 3β （GSK-3β） at 48h after treatment with different concentrations of Sch B were measured by activity assay kit. Results The Sch B can increase the proliferation inhibition rates in a dose-and time-dependent manner，and elevate the early and late apoptosis and cytoplasm／nucleus activities of GSK-3β at 48h after treatment. In addition to 1.0μmol／L， the proportion of cells in G0／G1phase of the remaining concentrations were higher than those of 0μmo／L （P＜0.05）， and the proportion of cells in S phase and G2／M phase and β-catenin，C-myc and Cyclin D1 protein levels were lower than those of 0μmo／L （P＜0.05）.Conclusion Sch B can inhibit the proliferation，promote apoptosis and cell cycle arrest and inhibit the activation of Wnt／β-catenin pathway.