Abstract: Objective To explore the expressions of miR-29c， miR-133b and miR-1 in ovarian cancer tissue and analyze the relationship between the above indicators and clinical pathology parameters of ovarian cancer. Methods Cancer tissue from 65 patients with ovarian cancer were collected as cancer group. The real-time quantitative PCR （qRT-PCR） was used to detect the expressions of miR-29c， miR-133b and miR-1 and the clinical pathology parameters of ovarian cancer （age， clinical stage， degree of differentiation， histological type and lymph node metastasis） were collected. Meanwhile， 32 cases of normal ovarian epithelial tissues （normal group） and 39 cases of benign ovarian cyst tissue （benign group） were taken as control. The average level of three indices of normal group were chosen as boundary value， and then the patients were categorized into high-level group （＞boundary value） and low-level group （≤boundary value）. The clinical pathology parameters of patients with different levels of miR-29c， miR-133b and miR-1 were compared. The relationships among miR-29c， miR-133b and miR-1 were investigated in cancer group. Results There were higher levels of miR-29c and but lower miR-133b and miR-1 in ovarian cancer group versus other two groups with significant difference （P＜0.05）. In ovarian cancer， the levels of three miRNAs were related with degree of differentiation， and miR-29c was related with clinical stage and lymph node metastasis， and miR-133b was related with lymph node metastasis （P＜0.05）. The level of miR-29c was negatively correlated with miR-133b and miR-1 （r=-0.541, r=-0.361）， and miR-133b was positively correlated with miR-1 with statistically significance （P＜0.05）. Conclusion There were higher expression of miR-29c but lower expression of miR-133b and miR-1. The levels of the above miRNAs were related with clinical pathology parameters in ovarian cancer tissue， showing a certain value in the diagnosis of ovarian cancer.