胃癌组织中幽门螺杆菌感染与HIF-2α、ABCG-2表达的相关性

临床肿瘤学杂志

胃癌组织中幽门螺杆菌感染与HIF-2α、ABCG-2表达的相关性

任盼盼1，冯玉光2，郭文君3，袁明亮3，桂忠娜3

1 261053 山东潍坊潍坊医学院研究生院 2 261042 潍坊医学院附属医院消化内科 3 261053 山东潍坊潍坊医学院研究生院

收稿日期:2015-07-01 修回日期:2015-07-23 出版日期:2015-09-30 发布日期:2015-09-30
通讯作者: 冯玉光

The correlation of Helicobacter pylori infection and expression of HIF-2α，ABCG-2 in gastric cancer tissues

REN Panpan， FENG Yuguang， GUO Wenjun， YUAN Mingliang， GUI Zhongna

Department of Postgraduate， Weifang Medical College， Weifang 261053， China

Received:2015-07-01 Revised:2015-07-23 Online:2015-09-30 Published:2015-09-30
Contact: FENG Yuguang

摘要/Abstract

摘要： 【摘要】目的检测幽门螺杆菌感染（Hp）与HIF-2α、ABCG-2蛋白在胃癌组织中的表达，探讨Hp感染在胃癌发生、发展中的干细胞机制及对化疗抵抗的分子机制。方法采用快速尿素酶试验和免疫组化SP法联合检测60例胃癌组织和45例慢性胃炎组织的Hp感染情况，免疫组化SP法检测胃癌组织中HIF-2α、ABCG-2蛋白的表达情况。结果胃癌组织中Hp的阳性表达率为67.3％（37／55），明显高于慢性胃炎组织的45.2％（1942），差异有统计学意义（P=0.029）。Hp感染与胃癌的浸润深度和淋巴结转移有关（P<0.05），而与性别、年龄、组织分化程度及远处转移无关（P＞0.05）。HIF-2α蛋白在Hp阳性胃癌组织与Hp阴性胃癌组织中的阳性表达率分别为81.1％和50.0％，且HIF-2α蛋白表达与Hp感染之间呈正相关（r=0.321，P=0.017）。ABCG-2蛋白在Hp阳性胃癌组织与Hp阴性胃癌组织中的阳性表达率分别为86.5％和55.6％，且ABCG-2蛋白表达与Hp感染之间呈正相关（r=0.342，P=0.011）。在37例Hp阳性胃癌组织中，HIF-2α与ABCG-2表达呈正相关（r=0.604，P＜0.0001）。结论 Hp可能通过激活HIF-2α-ABCG-2通路来促进胃癌组织中HIF-2α和ABCG-2的表达，诱导胃癌细胞的干细胞化及化疗抵抗。

Abstract: 【Abstract】Objective To study the helicobacter pylori（Hp）infection and the expression of hypoxiainducible factor-2α(HIF-2α）and ABCG-2 in gastric cancer tissues and discuss Hp infection in the initiation and development of gastric cancer as well as chemotherapy resistance induction. Methods Hp infection was identified by rapid urease test and immunocytochemistry in gastric cancer（n=60）and chronic gastritis（n=45）. Immunohistochemistry was used to detect the expression of HIF-2α and ABCG2 proteins in gastric cancer tissue. Results Hp positive rate of gastric cancer group（67.3％） was higher than that of chronic gastritis group（45.2％）with statistic significance（P=0.029）. Hp infection was correlated with the depth of infiltration and lymph node metastasis（P＜0. 05），but not with gender，age，tissue differentiation and distant metastasis（P＞0.05）. The positive rate of HIF2α protein in Hp（+）and Hp（-）gastric cancer tissues were 81.1％ and 50.0％. Hp infection and the expression of HIF-2α protein in gastric cancer tissues was positively correlated（r=0.321，P=0.017）. The positive rate of ABCG-2 protein in the Hp（+）and Hp（-）gastric cancer tissues were 86.5％ and 55.6％. Hp infection and the expression of ABCG-2 protein in gastric cancer tissues was positively correlated（r=0.342，P=0.011）. The expression of HIF-2α was positively correlated with ABCG-2 in Hp（+）gastric cancer tissue（r=0.604，P＜0.0001）. Conclusion Hp can stimulate the expression of HIF-2α and ABCG-2 in gastric cancer tissues by activating HIF-2α-ABCG-2 pathway，suggesting that Hp may play an important role in inducing stem cellularization of gastric cancer cells and chemotherapy resistance.

任盼盼1，冯玉光2，郭文君3，袁明亮3，桂忠娜3. 胃癌组织中幽门螺杆菌感染与HIF-2α、ABCG-2表达的相关性[J]. 临床肿瘤学杂志, 2015, 20(9): 808-.

REN Panpan， FENG Yuguang， GUO Wenjun， YUAN Mingliang， GUI Zhongna. The correlation of Helicobacter pylori infection and expression of HIF-2α，ABCG-2 in gastric cancer tissues[J]. Chinese Clinical Oncology, 2015, 20(9): 808-.

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