Abstract: 【Abstract】Objective To observe the effect of sorafenib，oxaliplatin alone or in different combination on the proliferation，migration and invasion of human hepatic carcinoma cell line HepG2. Methods Human hepatic carcinoma cell line HepG2 was chosen and divided into 6 groups： oxaliplatin group （0.5 μg／ml，O），sorafenib group（2.0 μmol／L，S），combination group（oxaliplatin 0.5 μg／ml，sorafenib 2.0 μmol／L； O+S），oxaliplatin sequent by sorafenib group （oxaliplatin 0.5 μg／ml，sorafenib 2.0 μmol／L；OS），sorafenib sequent by oxaliplatin group（sorafenib 2.0 μmol／L，oxaliplatin 0.5 μg／ml；SO），and the negative control group（100 μl common culture，C）. MTT method， immunofluorescence technique，flow cytometry， migration experiment，and invade experiment were used to observe sorafenib，oxaliplatin alone or in different combination on the proliferation， invasion and metastasis of HepG2 cells. Results The results determined by MTT method showed that the treatment groups significantly inhibited the proliferation of HepG2 cells after 48 h. Cell inhibition rate of O+S group was （72.13±0.99）％，which was significantly higher than that of O，S groups and SO group（P＜0.01）， except OS group. Flow cytometry and fluorescence microscopy analysis showed that the apoptosis rate of HepG2 cells in O+S group was the highest（65.33％）at 48 h，but it had no significant difference compared with OS group（P＞0.05）. Cell migration experiment showed that the inhibition rate of cell migration in O+S group was（71.67±6.66）％，which was significantly higher than that of O，S groups and SO group（P＜0.01），except OS group. O+S group and OS group exhibited synergistic effect of both sorafenib and oxaliplatin （P＜0.01），while SO group showed additive effect（P＜0.05）. Cells invade experiment showed that cell invasion inhibition rate of O+S group was（76.00±5.57）％，which was significantly higher than that of O，and S groups（P＜0.01）. There were two drugs synergistic effect in O+S group and OS group. Conclusion Compared with single drug of sorafenib or oxaliplatin，the inhibition of proliferation, migration and invasion of HepG2 cells treated by combination and sequential application of sorafenib，oxaliplatin are higher. The synergistic effect is similar between oxaliplatin sequent by sorafenib and sorafenib in combination with oxaliplatin，which are better than sorafenib sequent by oxaliplatin.