Abstract:

Objective To investigate the expression and significance of RIPK4 protein in human osteosarcomatous tissue and cells. Methods Imunohistochemistry was performed to detect the expression of RIPK4 protein in paraffin speciments of 30 osteosarcoma patients（and 10 osteochondroma patients. The correlation among clinicopathologic characteristics and RIPK4 protein was analyzed. The expression of RIPK4 mRNA in osteosarcoma MG-63， U-2OS and osteoblast cells was detected by RT-PCR， and differences were compared. Results The positive expression of RIPK4 in osteosarcoma was 60.0％（18/30）， while it was 20.0％（2/10） in osteochondroma.The differences were statistically significant（P＜0.05）. RIPK4 mRNA of osteoblast cells was 0.6500±0.2121， which was significantly lower than that of MG-63（1.6079±0.2661） and U-2OS（1.5000±0.1414），with singnificant differences（P＜0.05）. The expression of RIPK4 protein was related to TNM stage and lung metastasis（P＜0.05）. Conclusion The expression of RIPK4 protein and gene in osteosarcoma tissues and cells is high. RIPK4 may be involved in the progress of epithelial osteosarcoma.