Abstract:

Objective To investigate the relationship between helicobacter pylori（Hp） infection and expression level of survivin mRNA in the peripheral blood of gastric cancer patients. Methods The expression levels of survivin mRNA in the peripheral blood of gastric cancer and gastritis were detected by real-time quantitative reverse transcription polymerase chain reaction （qPCR）. Hp infection was determined by rapid urease test and giemasa staining. Results In the specimens of gastritis patients and gastric cancer patients，the expression rates of survivin mRNA were 0 and 84.0％. Median value was 0.46 for expression of survivin mRNA in the peripheral blood of gastric cancer patients. The expression levels of survivin mRNA were unrelated with age， gender and lymph node metastasis（P＞0.05）， but was significantly related with TNM stage（P＜0.05）. The positive expression rate of Hp in gastric cancer was 80.0％（40/50）， significantly higher than 58.0％（29/50） in gastritis patients（P＜0.05）. In the gastric cancer patients, the expression levels of survivin mRNA in Hp postive infection was 0.578±0.507， higher than 0.194±0.419 in Hp negative infection with significant differences （P＜0.05）. The expression survivin mRNA in gastric cancer patients and Hp infection was positively correlated（r=0.464， P＜0.05）. Conclusion The expression of survivin and Hp infection may be correlated with gastric cancer. Survivin may participate in the progress of gastric cancer with Hp infection.