Abstract:

Objective To explore the expression and clinical significance of lncRNA CADM1-AS1 in lung adenocarcinoma. Methods In this study, 72 cases of lung adenocarcinoma tissues and corresponding para carcinoma tissues were collected from our hospital. Real time quantitative PCR （qPCR） was used to detect the level of CADM1-AS1 in tissue samples. The relationship between CADM1-AS1 level and clinical pathological parameters （gender， age， tumor size， differentiation， clinical stage， T stage， smoking history and lymph node metastasis） was analyzed. The median overall survival （OS） was compared between different CADM1-AS1 levels according to the long-term survival data. The levels of CADM1-AS1 in common non-small cell lung cancer cells （NCI-H1299， SPC-A， NCI-H1650 and A549） were detected by qPCR assay. The effectiveness of CADM1-AS1 level in early diagnosis of lung cancer was evaluated by the receiver operating characteristic curve （ROC）. Results The level of CADM1-AS1 in lung adenocarcinoma tissues was lower than that in para carcinoma tissues （P＜0.05）. Compared with the normal bronchial epithelial cell line 16HBE，the CADM1-AS1 of the non small cell lung cancer was lower （P＜0.05）. CADM1-AS1 level was related to TNM stage， differentiation， tumor size， T stage and lymph node metastasis of lung adenocarcinoma （P＜0.05）. The median OS in patients with high CADM1-AS1 level was 32.5 months， higher than 16.0 months of patients with low CADM1-AS1 level （P＜0.05）. The value of CADM1-AS1 in the early diagnosis of lung adenocarcinoma was good with the area under the curve of 0.874， and the sensitivity and specificity were 87.8％ and 85.2％ respectively. Conclusion There were relative low levels of CADM1-AS1 in lung adenocarcinoma cell line and non-small cell lung cancer cell line. The CADM1-AS1 level is related to the TNM stage， differentiation and prognosis， which may be related to the occurrence and development of lung adenocarcinoma. It has a good value for early diagnosis of lung adenocarcinoma.