Abstract: Objective To investigate the expression and clinical significance of forkhead C2（FOXC2）and E-cadherin（E-cad）in colon cancer tissues. Methods From Jan 2014 to Mar 2016，70 cases of colon cancer and 30 adjacent normal tissues were collected. Immunohistochemical SP method was used to determine the expression of FOXC2 and E-cad in those tissues. The correlation of the two proteins in colon cancer tissues，and the relationship of the two proteins with pathological features were analyzed. Results The expression of FOXC2（74.3％，52／70） in colon cancer tissues was significantly higher than that in adjacent normal tissues（20.0％，6／30）with statistic significance（P＜0.001）. The expression of E-cad（21.4％，15／70）in colon cancer tissues was lower than that in adjacent normal tissues（80.0％，24／30）with statistic significance（P＜0.001）. The expression of FOXC2 and E-cad was related with TNM staging of tumor，depth of invasion and lymph node metastasis（P＜0.05），and no association was observed in age，gender，tumor size and histological grading（P＞0.05）. The two proteins were negatively correlated in colon cancer tissues（r=-0.728，P＜0.05）. Conclusion FOXC2 shows high expression in colon cancer tissues， while Ecad is low expressed. Meanwhile，a negative correlation is observed between the two proteins，suggesting that FOXC2 and E-cad may be involved in the occurrence and development of colon cancer.