Abstract: Objective To investigate the expression of transcription factor POU5F1 in colorectal cancer tissues and its relationship with clinicopathological features and prognosis. Methods One hundred and forty-two cases of paraffin-embedded colorectal cancer tissues and 86 cases of paraffin-embedded adjacent mucosa tissues were collected from January 2011 to July 2013. The expression of POU5F1 was measured by immunohistochemical SP method in 142 cases of colorectal cancer and 86 cases of adjacent mucosa tissues. The relationship of POU5F1 expression with clinicopathological parameters was analyzed. According to follow-up data，the prognosis of patients with different expression level of POU5F1 was investigated. The factors influencing prognosis was analyzed by Cox proportional hazard model. Results The positive expression of POU5F1 was localized in the nucleus and cytoplasm. The high expression rate of POU5F1 in colorectal cancer tissues was 47.2％（67／142），higher than 23.3％（20／86）of adjacent normal mucosa tissues，and the difference was statistically significant（P＜0.05）. The expression of POU5F1 was not related to sex，age，tumor location，distant metastasis，CEA，CA50 and CA199 of colorectal cancer，but it was related to the depth of invasion，lymph node metastasis，TNM stage and differentiation（P＜0.05）. The median overall survival（OS） was 26.4 months in patients with high expression，worse than 33.6 months in patients with low expression（P＜0.05）. Cox proportional hazard model analysis showed that the depth of invasion，lymph node metastasis，distant metastasis，TNM staging and POU5F1 expression were independent factors influencing OS. Conclusion The expression of POU5F1 in colorectal cancer tissues is associated with the degree of invasion，lymph node metastasis，TNM stage,differentiation and OS，and it has certain value in the diagnosis and prognostic prediction of colorectal cancer.