Abstract: Objective To explore the expression of cancer stem cell related genes Bmi1, POU5F1 in cervical cancer and their relationship with clinicopathological chracteristics. Methods The expression of Bmi-1， POU5F1 at mRNA level in 69 cervical cancer tissues and 16 cervical cancer tissues plus their paracarcinoma tissues were detected by realtime fluorescent quantitative PCR（QRT-PCR）. Results The expression levels of Bmi-1 mRNA， POU5F1 mRNA in cervical cancer tissues were 1.51±0.05 and 1.75±0.17， higher than 1.16±0.17 and 1.46±0.18 of their counterparts of the paracarcinoma tissues with statistically significant （P＜0.05）. The relative ratio of level of Bmi-1 mRNA， POU5F1 mRNA was 1.31±0.49 and 1.20±0.95 between cervical cancer tissues and their paracarcinoma tissues. The expression of Bmi1 mRNA was related with ethnic， lymph node metastasis， clinical staging and histological grade （P＜0.05），but not with age， tumor size and pathological type（P＞0.05）. The expression of POU5F1 mRNA was related with ethnic， tumor size， lymph node metastasis， clinical staging and histological grade （P＜0.05），but not with age and pathological type （P＞0.05）. Conclusion There was a relative high expression of Bmi-1， POU5F1 in cervical cancer. Bmi-1， POU5F1 expression may be associated with adverse biological characteristics of cervical cancer， and may serve as a potential indicator for molecular markers of cervical cancer biological behavior.