关键词: 胃癌, 白藜芦醇, 微小核糖核酸, 阿帕替尼, 敏感性

Abstract: Objective To investigate the effect and possible mechanism of resveratrol （RES） on the resistance of gastric cancer cell to apatinib. Methods  SGC7901 and SGC7901／AR cells were cultured in vitro. MTT assay and scratch test were used to detect the effects of different concentrations of apatinib or combined with RES on proliferation and migration of SGC7901 and SGC7901／AR cells. Resistance index， reversal multiple （RF） and relative reversal rate （RRR） of SGC7901 and SGC7901／AR cells were calculated respectively. Real-time fluorescence quantitative PCR （QPCR） was used to detect the miR-122， p-VEGFR2， p-Akt mRNA in these cells. Results Apatinib significantly inhibited the proliferation and migration of SGC7901 cells in a concentration-dependent manner （P＜0.05）， and the resistance index was 15.57. Apatinib combined with 50.0 μmol／L RES significantly inhibited the proliferation and migration of SGC7901 and SGC7901／AR cells in a concentration-dependent manner （P＜0.05）. The resistance index was 2.13， the reversal ratio of RES was 7.91 times， and the relative reverse rate was 93.4％. The expression levels of miR-122， p-VEGFR2， p-Akt mRNA in untreated SGC7901 cells were 0.74±0.11， 0.76±0.13 and 0.67±0.09， respectively， which were significantly higher than those in SGC7901／AR cells （P＜0.05）. The expression levels of p-VEGF R2 and p-Akt mRNA in SGC7901 cells decreased significantly after treatment with 10 μmol／L apatinib （P＜0.05）. After treatment with 10 μmol／L apatinib and 50.0 μmol／L RES， the expression of miR-122 in SGC7901 and SGC7901／AR cells increased significantly （P＜0.05）， while the expression of p-VEGFR2 and p-Akt mRNA decreased significantly （P＜0.05）. Conclusion Resveratrol can reverse the drug resistance of gastric cancer cell lines to apatinib， possibly by up-regulating the level of miR-122 and inhibiting the phosphorylation of VEGFR2 and Akt.

Key words: Gastric cancer, Resveratrol, MicroRNAs, Aapatinib, Sensitivity