Abstract: Objective To investigate the expressions of vascular endothelial growth factor receptor 2 （VEGFR-2） mRNA in different lung cancer cell lines. Methods The expressions of VEGFR2 mRNA in the human pulmonary mucoepidermoid carcinoma cell line NCI-H292， lung adenocarcinoma cell line NCI-H1299， lung adenocarcinoma cell line A549， lung giant cell carcinoma 95D and lung squamous carcinoma cell line Calu-1 were detected by real-time quantitative PCR （RT-PCR）. Results The expressions of VEGFR-2 mRNA were 1.213±0.134， 1.008±0.164，0.754±0.088， 0.582±0.171 and 0.121±0.026 in Calu-1，NCI-H292，95D，NCI-H1299 and A549 cell lines， showing significant differences （F=31.875， P=0.000）. No significant difference was observed in the expression of VEGFR-2 mRNA between Calu-1 and NCI-H292 （P=0.080） or between NCI-H1299 and 95D （P=0.137）. Conclusion The expression of VEGFR-2 mRNA is diverse in different lung cancer cell lines， which is closed with the different biological behavior.