Abstract: Objective To investigate the sensitivity of 22 kinds of chemotherapeutic drugs in patients bearing different maligrant tumors, in order to select proper drugs and provide reference for clinical application. Methods Malignant tumor cells of 182 samples were affected by 22 kinds of chemotherapeutic drugs for 2448 hours on the basis of clinical peak plasma concentation（PPC），and then the resistance rate and investigate the chemosensivity of antitumor drugs were tested by MTT assay. Results The resistance rate of 40 varieties of spongioblastoma cells affected by 7 different kinds of chemotherapeutic drugs was different in the condition of 1.0 PPC and 3-5×105／ml cell concentration. BCNU，TMZ and VM26 of which have higher resistance rate of malignant tumors than the other chemotherapeutic drugs. The 7 different kinds of chemotherapeutic drugs classified in susceptibility rate from high to low was BCNU＞TMZ＞DDP＞VM26＞VCR＞CBP＞VP16.The resistance rate of 59 varieties of oophoroma cells affected by 13 different kinds of chemotherapeutic drugs was different, and the susceptibility rate of these 13 different chemotherapeutic drugs classified from high to low was L-OHP＞TPT＞TAX＞ADM＞TXT＞GEM＞CBP＞CTX＞IFO＞LBP＞DDP＞PYM＞EADM. The resistance rate of 19 varieties of bone and soft tissue tumor cells affected by 6 different kinds of chemotherapeutic drugs was similarity，of which MTX and TAX have the resistance rate more than 40％. These chemotherapeutic drugs classified in susceptibility rate from high to low was ADM＞MTX＞IFO＞DDP＞TAX＞DTIC. The resistance rate of 64 varieties of head and neck cancer cells affected by 8 different kinds of chemotherapeutic drugs was different, and the susceptibility rate of these chemotherapeutic drugs classified from high to low was PYM＞CTX＞BLM＞DDP＞5-FU＞VP16＞VCR＞TAX. Conclusion Malignant tumor patients have significant individual differences to chemotherapeutic drugs in chemosensitivity. The individual screening of chemosensitivity to different kinds of cancer patients before chemotherapy application is absolutely essential. It can decrease toxic reaction by avoiding blindly using chemotherapeutic drugs and increase therapeutic effect and survival quality. Chemotherapy under the guidance of chemosensitivity test in vitro may improve the therapeutic effect and help clinician to choose reasonable chemotherapy drugs for patients individually.