Abstract: Objective To explore the expressions of Ski-novel protein （SnoN）， early growth response protein 3 （Egr3） and secreted frizzled related protein 1（SFRP1） in gastric cancer tissue，and further analyze their clinicopathological characteristics in gastric cancer. Methods Seventy-five cases of gastric cancer tissues and 62 cases of para-cancer tissues were collected. The immunohistochemical Elivision method was used to analyze the expressions of SnoN，Egr3 and SFRP1 from the above tissues. The clinicopathological characteristics of gastric cancer patients were collected and subsequently analyzed for their correlation with expressions of SnoN，Egr3 and SFRP1. Results The high-level expression rates of SnoN and SFRP1 were 45.3%（34/75） and 38.7%（29/75）， both lower than 62.9%（39/62） and 58.1%（36/62） in para-cancer tissues （P＜0.05）. While the high-level expression rate of Egr3 was 69.3%（52/75） in cancer tissues， higher than 38.7%（24/62） in para-cancer tissues （P＜0.05）. The expression of SnoN， Egr3 and SFRP1 were related with clinical stage and degree of differentiation. In addition，the SnoN expression was related with age，depth of invasion and lymph node metastasis，and SFRP1 expression was related with age， tumor size and depth of invasion. The expressions of both SnoN and SFRP1 were all negatively correlated with Egr3 in cancer tissues （r=-0.324，-0.244） with significant difference （P＜0.05）. The SnoN expression was positively correlated with SFRP1（r=0.487， P＜0.001）. Conclusion There are high expression of Egr3 but low expression of SnoN and SFRP1 in gastric cancer. All tested proteins are related with clinical stage and degree of differentiation， indicating an important role in the occurrence and development of gastric cancer.