Abstract:

Objective To investigate the expression of 6-phosphofructo-2kinase／fructose-2，6-bisphosphatase （PFKFB3） in hepatocellular carcinoma（HCC） and its clinical significance. Methods The expressions of PFKFB3 mRNA of tissue sections from 42 HCC and paired adjacent tissues were detected by RT-PCR. Then the relationships between PFKFB3 expression and clinicopathological variables （gender， age， tumor size， lymphatic metastasis， cancer embolus in vein， AFP level before treatment， differentiation and metastasis in liver） were analyzed. Meanwhile， the difference of overall survival of patients with different expression of PFKFB3 were studied by survival analysis of Kaplanmeier. Results The positive expression rate and relative level of PFKFB3 in HCC tissue were 73.81％ （31／42） and 1.075±0.171， higher than 52.38％ （22／42） and 0.877±0.451 in adjacent tissues with significant difference （P＜0.05）. The PFKFB3 expression is related with TNM stage， degree of ifferentiation， intrahepatic metastasis and overall survival （P＜0.05）. Conclusion PFKFB3 were related to carcinogenesis， development and metastasis of HCC. The expression of PFKFB3 can influence the differentiation， metastasis andprognosis of HCC， indicating it may be used as biological indicators to evaluate the degree of malignancy of HCC.