Abstract: Objective To explore the radiosensitization effect of curcumin plus cisplatin on non-small cell lung cancer A549 cells. Methods Cell viability at 24，48 and 72 h after treatment with different concentrations （10，20，50，100，200 μmol/L） of curcumin or（1， 2， 5，10，20 mg/L） of cisplatin were determined by MTT. According to the experimental protocol， the below experiments were carried out in irradiation （R） group， curcumin+irradiation （C+R） group， cisplatin+irradiation （P+R） group and curcumin+cisplatin+irradiation （C+P+R） group. The colony formation assay was employed to observe the surviving fraction （SF） of above four groups after X-ray irradiation of 0， 2，4，6，8，10 Gy. The artificial scratch， Transwell test and Western blotting were employed to detect the cell migration， invasion and protein level of epidermal growth factor receptor （EGFR） at 24 h after treatment in four groups. Results The cell viability of A549 cells gradually decreased with the increasing concentration of curcumin ranging from 10 to 200 μmol/L and cisplatin ranging from 1 to 20 mg/L in a dose- and time-dependent manner （P<0.05）. The SF of C+P+R group were lower than the remaining 3 groups under the dose of 2-10 Gy （P<0.05）. Compared with the R irradiation group， there was lower SF in C+R group under the dose of 4-10 Gy and P+R group under the dose of 2-10 Gy with significant difference （P<0.05）. Compared with R group, the sensitizing enhancement ratio were 1.24， 1.31 and 1.96 in C+R group， P+R group and C+P+R group， respectively. There were lower migration distance， transmembrane cell number and EGFR protein level in C+P+R group versus the remaining 3 groups （P<0.05）. Compared with the R group， the above indicators were also lower in C+R group and P+R group under the dose of 2-10 Gy with significant difference （P<0.05）. Conclusion Curcumin plus cisplatin can inhibit the proliferation of A549 cells with radiosensitizing effect and suppress its migration and invasion possibly via inhibition of EGFR signaling pathways.