Abstract: Objective To explore the levels of plasma miR-205， miR-212 and miR-429 in ovarian cancer and analyze the relationship between the above indicators and clinical pathology parameters of ovarian cancer. Methods Plasma samples from 71 ovarian cancer patients before treatment were collected. The real-time quantitative RT-PCR （qRT-PCR） was used to detect the levels of miR-205， miR-212 and miR-429， and the clinical pathology parameters of ovarian cancer （age， clinical stage， degree of differentiation， histological type and lymph node metastasis） were collected. The clinical pathology parameters of patients with different levels of miR-205， miR-212 and miR-429 were compared. The receiver operating characteristic curve （ROC） was employed to analyze the clinical value of plasma miR-205， miR-212 and miR-429 in the diagnosis of ovarian cancer. Meanwhile， the plasma samples from 68 healthy volunteers （healthy control group） and 66 patients with benign ovarian tumor （benign group） were collected as control. Results There were higher levels of miR-205 and but lower miR-212 and miR-429 in ovarian cancer group versus other two groups with significant difference （P all<0.05）. In ovarian cancer， the level of miR-205 was related with age， clinical stage， degree of differentiation and lymph node metastasis， and miR-212 was related with clinical stage and degree of differentiation， and miR-429 was related with clinical stage and lymph node metastasis （P all<0.05）. The plasma level of miR-205 was negatively correlated with miR-212 and miR-429 （r=-0.572、-0.325）， and miR-212 was positively correlated with miR-429 with statistically significance （r=0.473，P<0.05）. The AUC， sensitivity and specificity of plasma miR-205， miR-212 and miR-429 in the diagnosis of ovarian cancer were 0.915， 92.1% and 86.2%， 0.944，87.3% and 91.0%， and 0.905， 88.5% and 83.6%. Conclusion There was higher expression of miR-205 but lower expression of miR-212 and miR-429. The plasma levels of the above indicators were related with clinical pathology parameters， showing a certain value in the diagnosis of ovarian cancer.