Abstract: Objective To investigate the expression levels of nuclear receptor 5A2（nr5A2）and Glypican-3（GPC3）in gastric cancer tissues and the relationship between them and the clinical pathological characteristics and prognosis of gastric cancer. Methods The expression of GPC3 and nr5A2 in paraffin sections of 68 cases of gastric cancer tissues and 59 cases of para carcinoma tissues were detected by immunohistochemical SP method. According to product value of the percentage of positive cells and intensity of staining， the expression levels were divided into negative expression（＜3） and positive expression（≥3）. We analyzed the relationship between the different expression and clinical pathological parameters（gender， age， clinical stage， tumor size， Lauren type， differentiation degree， invasion depth and lymph node metastasis）and prognosis of gastric cancer. Spearman correlation analysis was used to analyze the expression of GPC3 and nr5A2 in gastric cancer tissues， and the prognosis of patients with different expression of the both proteins was analyzed by KaplanMeier method. Results The positive expression rates of nr5A2 and GPC3 in gastric cancer tissues were 64.7％（44／68）and 57.4％（39／68）， higher than 32.2％（19／59）and 22.0％（13／59）of the adjacent tissues with statistical significance（P＜0.05）. The expression of nr5A2 was positively correlated with GPC3 in gastric cancer tissues（r=0.421， P=0.001）. The expression of the both proteins was related to clinical stage and Lauren typing. In addition， the expression of nr5A2 was related to the depth of invasion， and the expression of GPC3 was related to the degree of differentiation and lymph node metastasis（P＜0.05）. The median survival time（OS）of nr5A2 positive expression patients was 22.5 months， lower than 32.0 months of nr5A2 negative expression patients（P＜0.05）. The difference was not statistically significant between different expression levels of GPC3. Conclusion Both nr5A2 and GPC3 are positive expression in gastric cancer tissues， which is related to clinical stage and Lauren typing. nr5A2 expression is related to prognosis. Both proteins may play an important role in the development of gastric cancer， which can be used in the diagnosis and evaluation of gastric cancer.