Abstract: Objective To investigate the expression and clinical significance of Cyclin A， PTEN and p27kip1 in esophageal squamous cell carcinoma （ESCC）.
MethodsThe expression of Cyclin A， PTEN and p27kip1 proteins were detected by the immunohistochemical En Vision method in 68 ESCC specimens and 25 tumoradjacent normal tissues. Results The positive expression rates of Cyclin A， PTEN and p27kip1 in esophageal squamous cell carcinoma were 60.3％， 35.3％， 41.2％，respectively，which had statistically significant difference with those of normal tissues （P＜0.05）. The expression of Cyclin A， PTEN and p27kip1 proteins were correlated with histological grade， lymph node metastasis and tumor size（P＜0.05）.Cyclin A expression was associated with age（P＜0.05）.The results of Kaplan-Meier survival analysis indicated that the positive expression of Cyclin A and the negative expression of PTEN and p27kip1were associated with a considerably worse survival. Conclusion Cyclin A， PTEN and p27kip1may play a vital role in ESCC development，metastasis and prognosis. Detection of the expression of Cyclin A， PTEN and p27kip1 is valuable in evaluating of the biological behavior and prognosis of ESCC.