Abstract:  

Objective  To investigate the expression of microRNA-1253（miR-1253） in lung adenocarcinoma cell lines and its effect on the proliferation， migration and invasion of lung adenocarcinoma cells. Methods  Real-time fluorescence quantitative PCR （QPCR） was used to detect the expression of miR-1253 in lung adenocarcinoma cell lines A549， NCI-H1299， NCI-H157， A973 and GLC-82. miR-1253 mimics and miR-1253 inhibitor were transfected into A973 and NCI-H157 cells respectively. The cells transfected with negative control plasmid NC were used as negative control（NC） group. The effects of different expression of miR-1253 on proliferation， clone formation， invasion and migration of A973 and NCI-H157 cells were examined by cell proliferation assay, clone formation assay and Transwell migration and invasion assay. Results  The results of QPCR showed that the relative expression of miR-1253 in A549， NCI-H1299， NCI-H157， A973 and GLC-82 cells were 0.92±0.06, 0.06±0.03, 1.10±0.26, 0.03±0.01 and 0.45±0.08， respectively. After transfection of miR-1253 mimics into A973 cells， the relative expression of miR-1253 increased significantly（P＜0.05）， and decreased significantly after transfection of miR-1253 inhibitor into NCI-H157 cells （P＜0.05）. Compared with the NC group， the transfection of miR-1253 mimics could significantly inhibit the proliferation activity， the ability of plate cloning（166.0±29.3 vs. 371.0±31.4， P=0.001） and the ability of migration（91.1±32.1 vs. 166.7±33.9， P=0.008） and invasion（74.4±20.5 vs. 145.6±28.8，P=0.001） of lung adenocarcinoma cell line A973； miR-1253 inhibitor can up-regulate the proliferation， number of plate cloning cells（545.0±61.9 vs. 337.0±39.7， P=0.008）， migration（246.7±36.7 vs. 151.1±32.9，P＜0.001） and invasion（231.1±38.8 vs. 137.8±27.3， P=0.001） of NCI-H157. Conclusion  miR-1253 could reduce malignant phenotype of lung adenocarcinoma cells， and therefore it can be used as an effective target for gene therapy of lung adenocarcinoma.

Key words:  , Lung adenocarcinoma

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