Abstract: ObjectiveTo investigate the effect of microRNA933 （miR933） on the regulation of brainderived neurotrophic factor （BDNF） and the migration and invasion of hepatoma cells. 
MethodsThirty pairs of hepatoma and paracancerous tissues surgically removed in our hospital were collected from January 2017 to December 2017. Levels of miR933 in the above tissues and hepatoma cells （Huh7， HepG2 and SKHep1） were detected by realtime quantitative PCR （QPCR）. SKHep1 cells were transfected with miR933 mimic （overexpressing group） and negative control （control group） by liposome method. QPCR was used to detect the miR933 level of each group at 48 h after transfection to evaluate the transfection efficiency. The invasion and migration of each group were evaluated by Transwell and scratch test， respectively. QPCR and Western blotting were used to detect the mRNA and protein levels of BDNF in each group. The dual luciferase reporter gene assay was applied to confirm the target and binding sites between miR933 and BDNF. 
ResultsThe level of miR933 was 0351±0026 in hepatoma tissues， lower than 1124±0054 of normal paracancerous tissue （P＜005）. The levels of miR933 in Huh7， HepG2 and SKHep1 cells were 0751±0062， 0453±0057 and 0017±0006， lower than that of the healthy hepatocyte line L02 （P＜005）. The miR933 level of SKHep1 cells in overexpression group at 48 h after transfection was 3197±0354， higher than 1019±0079 of the control group （P＜005）. The rate of healing and the number of membrane cells in the overexpressed group were （244±39）％ and 576±68， lower than （625±47）％ and 1026±95 of the control group （P＜005）. The mRNA and protein levels of BDNF in the overexpression group were lower than those in the control group （P＜005）. The results of double fluorescence report showed that miR933 significantly inhibited the luciferase activity of cells transfected with the wild type BDNF3’UTR plasmid， but had no significant effect on cells transfected with mutant BDNF3’ UTR. 
ConclusionThe levels of miR933 in the tissues and cells of liver cancer were reduced， and the invasion and migration of hepatoma cells could be inhibited. It may be realized by targeting BDNF， which can be used as a potential target for the treatment of liver cancer.

Key words: Hepatic carcinoma, MicoRNA933, Invasion, Migration, Brainderived neurotrophic factor（BDNF）