Abstract: Objective To detect the expressions of novel oncogene with kinasedomain（NOK） and fibroblast growth factor receptor 2（FGFR2） protein in nonsmall cell lung cancer（NSCLC）， and analyze the expressions among different pathological types， pathological grades， TNM stages and their correlation. Methods The expressions of NOK and FGFR2 protein were detected with immunohistochemical En Vision method in 163 NSCLC samples， the expressions among different pathological types， pathological grades， TNM stages and the correlation between expressions of NOK and FGFR2 in tumor samples were analyzed. Results The positive rate of NOK and FGFR2 protein in 163 NSCLC tissues were 66.7％ and 852％，respectively. The positive rate of NOK protein in lung squamous cell carcinoma and adenocarcinoma were 64.5％ and 72.6％ with no statistical difference（P＞0.05）. NOK protein was expressed among different TNM stages with statistical difference（P=0.000），and the expressions also different in pathological grades（P=0.000）. The positive rate of FGFR2 protein in lung squamous cell carcinoma and adenocarcinoma were 886％ and 85.7％ with no statistical difference（P＞0.05）. The expression of FGFR2 protein at different TNM stages and different pathological grades were also different statistically（P=0.000）. The positive correlation between NOK and FGFR2 expression was found in NSCLC samples（r=0.640，P=0.000）， squamous cell carcinoma（r=0.684，P=0.000） and adenocarcinoma（r=0.597，P=0.00）. Conclusion NOK and FGFR2 protein are highly expressed in NSCLC， and their expressions correlates with pathological types， pathological grades and TNM stages， which may be related to the occurrence and development of NSCLC.