Abstract: Objective To investigate the correlation between the expression of lactate dehydrogenase A（LDH-A） and histone deacetylase 1（HDAC1） in intestinaltype gastric cancer（ITGC） and analyze the relationship between their expressions and prognosis. Methods The LDH-A siRNAexpressing lentiviral vector was constructed and used to transfect into ITGC cell line SGC7901. Western blotting was applied to detect the expression of HDAC1 protein. The expressions of LDH-A and HDAC1 in 661 ITGC specimens and the corresponding noncancerous tissues were performed by immunohistochemistry technique. Results According to Western blotting results， LDH-A expression was significantly upregulated in SGC7901 cells and the knockdown of LDH-A in SGC7901 cells significantly downregulated the expression of HDAC1. Based on the analysis of immunohistochemistry results， the high expression rate of LDH-A protein in ITGC was 54.8％（362／661），significantly higher than 12.9％（85／661）in paired adjacent noncancerous tissues（P＜0.01）； the high expression rate of HDAC1 protein in ITGC was 51.3％（339／661）， significantly higher than 15.4％（102／661） in paired adjacent noncancerous tissues（P＜0.01）； LDHA expression was positively related to HDAC1 expression（r=0.324， P＜0.001）. Univariate analysis showed that the survival curve of patients with low LDH-A／low HDAC1 expression was significantly better than that of other combinations in ITGC（P＜0.001）. Multivariate survival analysis showed that LDHA and HDAC1expression were independent prognostic factors.
ConclusionThe expressions of LDH-A and HDAC1 have positive correlation in ITGC. Targeted inhibition of both LDH-A and HDAC1 is promising to bring longer overall survival.