Abstract: Objective To investigate the expression of C17orf76-AS1 between normal ovarian epithelial cell and epithelial ovarian cancer cells and the effect of C17orf76-AS1 overexpression on the biological function of SKOV3 cells. Methods The quantitative real-time PCR（QPCR）was used to detect the expression of C17orf76-AS1 in normal ovarian cell （IOSE80） and ovarian cancer cells （A2780，SKOV3，OVCAR3，HO-8910 and HO-8910PM）. Construction of C17orf76-AS1 overexpression plasmid based on pcDNA3.1 plasmid and transient transfection of ovarian cancer cell SKOV3 with Lipo2000 were carried out. QPCR was used to detect the overexpression efficiency of C17orf76-AS1 in SKOV3 cells. CCK-8 was used to detect the proliferation of SKOV3.Transwell and wound healing assay were used to detect the migration of SKOV3. Results Compared with IOSE80， C17orf76-AS1 showed low expression in A2780，SKOV3，OVCAR3，HO-8910 and HO-8910PM about 0.36，0.30，0.46，0.32 and 0.31 folds（P＜0.05）. The proliferation of SKOV3 cells significant decreased about 1.5 folds after C17orf76-AS1 overexpression with CCK-8 assay. The Transwell assay demonstrated C17orf76-AS1 overexpression significant decreased the migration of SKOV3 by about 1.3 times， and the wound healing assay confirmed that the healing ability of SKOV3 was significantly lower than that of the control group after the overexpression of C17orf76-AS1（P＜0.05）. Conclusion LncRNA C17orf76-AS1 was downregulated in ovarian cancer cells. The overexpression of C17orf76-AS1 significant decreased the proliferation and migration of ovarian cancer cell.