Abstract:

Objective  Effects of isocryptotanshinone （ICTS） on proliferation， cycle and apoptosis of gastric cancer cell lines BGC-823 and SGC-7901 and its possible mechanism. Methods Gastric cancer cell lines BGC-823 and SGC-7901 were cultured in vitro. After treatment of 0，5，10，20 μmol／L ICTS for 24， 48 and 72 hours， CCK-8 method was used to detect the inhibition rate of cell proliferation. After determining the optimal concentration and time of ICTS inhibiting BGC-823 and SGC-7901 cells， cell cycle and apoptotic rate were detected by flow cytometry. Western blotting was used to detect the expression of Cyclin D1， Bcl-2， p53 and p21 proteins in gastric cancer cells treated with ICTS. Results The inhibition rates of BGC-823 cells treated with 0，5，10，20 μmol／L ICTS for 24 hours were （0.789±0.048）％， （16.74±1.55）％， （33.58±2.26）％ and （54.62±2.61）％. The difference was statistically significant （P＜0.05）. The inhibition rates of SGC-7901 cells treated with 0，5，10，20 μmol／L ICTS for 24 hours were （-0.184±0.023）％， （12.76±1.73）％， （32.95±2.47）％ and （53.80±2.65）％. The difference was statistically significant （P＜0.05）. The inhibition rates of BGC-823 cells treated with 20 μmol／L ICTS at 24， 48 and 72 hours were （54.62±2.61）％， （55.08±2.35）％， （59.72±2.53）％. There was no significant difference （P＞0.05）. The inhibition rates of SGC-7901 cells treated with 20 μmol／L ICTS at 24， 48 and 72 hours were （53.80±2.65）％，（55.76±2.47）％ and （61.83±2.82）％，. There was no significant difference （P＞0.05）. BGC-823 and SGC-7901 cells were treated with 20 μmol／L ICTS for 24 hours. The percentage of G0／G1 phase cells was （78.34±7.13）％ and （79.57±7.34）％， which were higher than those of the control group（P＜0.05）. The apoptotic rates of BGC-823 and SGC-7901 in ICTS treatment group were （24.78±3.42）％ and （28.76±4.21）％， which were higher than those of control group （P＜0.05）. The expression of Cyclin D1 and Bcl-2 protein in ICTS treatment group was significantly lower than that in the control group （P＜0.05）， and the expression of p53 and p21 protein in ICTS treatment group was significantly higher than that in the control group （P＜0.05）. Conclusion ICTS plays an anti-gastric cancer role by increasing the expression of p53 and p21， reducing the expression of Cyclin D1 and Bcl-2， thereby inhibiting cell proliferation， increasing G0／G1 blockade and apoptosis.

Key words:

Gastric cancer')">"> Gastric cancer, Isocryptotanshinone（ICTS）, Proliferation, Apoptosis