Abstract: Objective To explore the effect of NVP-BEZ235 on the proliferation， apoptosis， invasion and metastasis of HepG2 Cells. Methods The HepG2 cells were treated with different concentrations of NVP-BEZ235（0， 0.01， 0.1， 1.0μmol／L）. MTT was used to study the proliferation inhibition rates at 24， 48， 72 and 96h treated with different concentrations of NVP-BEZ235. Transwell assay was used to check the invasion and metastasis of HepG2 cells with NVP-BEZ235. The cycle distribution at 48h after treatment with NVP-BEZ235 was detected by flow cytometry. The RT-PCR and Western blotting were used to measure the change of MMP-2 expression. ResultsNVP-BEZ235 can increase the proliferation inhibition rates of HepG2 cell in a dose-and time-dependent manner. In addition to the late apoptosis rate of 0.01μmol／L at 24h，the early and late apoptosis rates of the remaining concentrations were higher than those of 0μmo／L at 24h and 48h（P＜0.05）. The proportion of cells in G0／G1 phase of 0.01， 0.1 and 1.0μmol／L NVP-BEZ235 were higher than those of 0μmo／L，while the transmembrane cell number， the proportion of cells in G2／M and S phase， and the protein and mRNA levels of MMP-2 of 0.01， 0.1 and 1.0μmol／L NVP-BEZ235 were lower than those of 0μmo／L at 48h（P＜0.05）.Conclusion NVP-BEZ235 effectively inhibits the abilities of proliferation， invasion and metastasis abilities of HepG2 cells， promotes the apoptosis， arrests cells at G0／G1 phase and decreases the expression of MMP-2.