Abstract: Objective To investigate the expression and relationship of HER2 and Ki67 in gastric cancer， and analyze their correlation with clinicopathological parameters. Methods FISH and immunohistochemical staining were used to detect the HER-2 gene amplification and Ki-67 protein expression in 156 gastric cancer tissues. The association between HER-2, Ki-67 expressions and clinicopathological features was analyzed. The interrelationship of HER-2 and Ki-67 expression in gastric cancer tissues was explored.
ResultsThe amplification rate of HER-2 gene was 11.5％（18／156）. The amplification rate of HER-2 gene was significantly related with depth of invasion and cancer embolus（P＜0.05）. The positive expression rate of Ki67 in gastric cancer tissues was 73.72%（115/156）, which was higher than 2.56%（4/156） of paracarcinoma tissues with significant differences（P＜0.05）. The expression of Ki-67 was significantly related with histological type and depth of invasion. HER-2 amplification and Ki-67 expression was positively correlated（r=0.162, P＜0.05）. Conclusion HER-2 and Ki-67 expression may be involved in the tumor progression of gastric cancer. The combination detection of HER-2 amplification and Ki-67 expression may be used to evaluate the patients'prognosis and develop an individualized therapy.