Abstract: Objective To investigate the expression and clinical significance of S-phase kinase-associated protein 2（Skp2） and p27 in the esophageal carcinoma. Methods Immunohistochemistry method was used to detect expression of Skp2 and p27 in 82 cases of esophageal carcinoma and 10 cases of normal esophageal epithelial tissue adjacent to esophageal cancer. The correlation between expressions of Skp2 and p27 protein with clinicopathological features was analyzed by chi-square test. Correlation analysis was made by Spearman correlation analysis. Results Positive rates of Skp2 and p27 were 67.07%（55/82） and 35.37%（29/82） in esophageal carcinoma, significant different from 20.00%（2/10） and 90.00%（9/10） in normal tissues（P＜0.01）. Skp2 expression was not associated with gender， age and tumor site， but with differentiation grades， the depth of tumor invasion， lymph node metastasis and clinical TNM stages（P＜0.05）； p27 expression was not associated with gender， age， tumor site， the depth of tumor invasion and clinical TNM stages， but with differentiation grades and lymph node metastasis（P＜0.05）.There was a negative correlation between Skp2 and p27 expression（r=-0.750，P＜0.001）. Conclusion Skp2 and p27 expression involve in the development and progression from normal esophageal tissue to esophageal carcinoma，and may be correlated to the onset and progress of esophageal carcinoma.