Abstract: Objective To investigate the effects of miR-143 on proliferation and apoptosis of renal cell carcinoma GRC-1 cells and its target hypoxia inducible factor-1α （HIF-1α）.
MethodsRenal cell carcinoma cell line GRC-1 cells were transfected with miR-143 mimics， and real time quantitative polymerase chain reaction （qPCR） was performed to evaluate the efficiency of transfection. According to the experiment， the GRC-1 cells were divided into 3 groups： non-transfection group， miR-143 control group and miR-143 transfection group. MTT method was used to detect the proliferation of cells in each group. Flow cytometry was used to detect the apoptosis of each group. The qPCR and Western blotting were employed to measure the mRNA and protein level of HIF-1α. Dual luciferase reporter gene was applied to verify the relationship between miR-143 and HIF-1α. Results The level of miR-143 in miR-143 transfection group was higher than those in non-transfection group and miR-143 control group， and the difference was statistically significant （P＜0.05）. Compared with other two groups， there were decreased proliferation rates but increased apoptotic rates in miR-143 transfection group （P＜0.05）. Transient overexpression of miR-143 in GRC-1 cells decreased the expression of HIF-1α on both mRNA and protein levels. Moreover， dual-luciferase reporter assay confirmed that HIF-1α was a direct target of miR-143 in GRC-1 cells.
ConclusionmiR-143 may regulate the proliferation of GRC-1 cells， and HIF-1α is a direct target of miR-143.