脑胶质瘤组织中微小RNA21水平及其与EGFR和VEGF表达的关系#br#

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摘要/Abstract

摘要： 目的探讨脑胶质瘤组织中微小RNA21（miR21）水平及其与表皮生长因子受体（EGFR）和血管内皮生长因子（VEGF）表达的关系。
方法选取本院2005年1月至2015年6月手术切除并经病理证实的WHO Ⅲ、Ⅳ级脑胶质瘤组织92例，另取50例癌旁组织作对照。采用实时荧光定量PCR（QPCR）检测以上组织中的miR21水平，免疫组化检测EGFR和VEGF表达，分析脑胶质瘤组织中miR21水平与临床病理参数（性别、年龄、民族和病理分级）及EGFR和VEGF表达的关系，Spearman等级相关分析miR21水平与EGFR和VEGF表达的相关性。
结果QPCR检测结果显示脑胶质瘤组织中miR21水平为1307±769，高于癌旁组织，差异有统计学意义（P＜005）；miR21水平与性别、年龄及民族无关（P＞005），仅与病理分级有关（P＜005），其中Ⅳ级的miR21水平为16483±4980，高于Ⅲ级的3229±3165，差异有统计学意义（P＜005）。EGFR和VEGF阳性表达率均为870％（80／92），其中EGFR阳性表达者的miR21水平为13952±8062，高于阴性表达者的3487±3309（P＜005），而VEGF阳性表达者的miR21水平为13874±8340，高于阴性表达者的2090±1458（P＜005）；Spearman等级相关分析发现脑胶质瘤组织中miR21水平与EGFR、VEGF表达均呈正相关（r=0251、0311， P＜005）。
结论脑胶质瘤组织中miR21水平升高，且与病理分级和EGFR、VEGF表达有关，可能参与了该恶性肿瘤的发生发展，对脑胶质瘤的诊治有一定临床意义。

关键词: 脑胶质瘤, 微小RNA21, 表皮生长因子受体, 血管内皮生长因子

Abstract: ObjectiveTo investigate the level of microRNA21（miR21） in glioma tissues and its relationship with the expressions of epidermal growth factor receptor（EGFR） and vascular endothelial growth factor（VEGF）.
MethodsNinetytwo cases of glioma tissues（WHO Ⅲ and Ⅳ grade） surgically resected and confirmed by pathology in our hospital from January 2005 to June 2015 were collected. Another 50 cases of paracancerous tissues were taken as control. Realtime quantitative PCR（QPCR） was used to detect the level of miR21 in the above tissues. Immunohistochemistry was used to detect the expressions of EGFR and VEGF. The relationships between miR21 level and clinicopathological parameters（gender， age， nationality and pathological grade） as well as the expressions of EGFR and VEGF were analyzed in glioma tissues. Correlation between miR21 level and expressions of EGFR and VEGF was analyzed by Spearman rank correlation analysis.
ResultsQPCR detection showed that the miR21 level in glioma tissues was 1307±769， higher than that in adjacent tissues， and the difference was statistically significant（P＜005）. The level of miR21 was not related to sex， age and nationality（P＞005）， only related to the pathological grade（P＜005）. The level of miR21 of grade Ⅳ was 16483±4980， higher than 3229±3165 of grade Ⅲ， and the difference was statistically significant（P＜005）. The positive expression rates of EGFR and VEGF were both 87％（80／92）. The miR21 level in EGFR positive ones was 13952±8062， higher than 3487±3309 of the negative ones（P＜005）； the miR21 level of the VEGF positive ones was 13874±8340， higher than 2090±1458 of the negative ones（P＜005）. Spearman rank correlation analysis showed that the miR21 level in glioma tissues was positively correlated with the expressions of EGFR and VEGF（r=0251， 0311， P＜005）.
ConclusionThe level of miR21 in glioma tissues was elevated， which was related to the pathological classification and the expression of EGFR and VEGF. It may be involved in the development of the malignant tumor， and it has certain clinical significance for the diagnosis and treatment of glioma.

Key words: Glioma, MicroRNA21, Epidermal growth factor receptor, Vascular endothelial growth factor

张照楠, 宿伟鹏, 张洋, 张宋安, 赵化荣. 脑胶质瘤组织中微小RNA21水平及其与EGFR和VEGF表达的关系#br#

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[J]. 临床肿瘤学杂志, 2018, 23(6): 514-518.

ZHANG Zhaonan, SU Weipeng, ZHANG Yang, ZHANG Songan, ZHAO Huarong. . Analysis of microRNA21 level in glioma tissues and its relationship with expressions of EGFR and VEGF[J]. Chinese Clinical Oncology, 2018, 23(6): 514-518.

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