EGFR和TGF-α在肺癌细胞放疗增敏中的作用

临床肿瘤学杂志 ›› 2019, Vol. 24 ›› Issue (2): 145-148.

EGFR和TGF-α在肺癌细胞放疗增敏中的作用

1 226361 江苏南通南通大学附属肿瘤医院放疗科

2 226361 南通大学附属肿瘤医院病理科

收稿日期:2018-07-14 修回日期:2018-11-22 出版日期:2019-02-28 发布日期:2019-03-18
基金资助:
基金项目：2016年南通市科技计划资助项目（YYZ16023）

The effect of EGFR and TGF-α in radiosensitization of lung cancer cells

Department of Radiotherapy， Cancer Hospital Affiliated to Nantong University， Nantong 226361， China

Received:2018-07-14 Revised:2018-11-22 Online:2019-02-28 Published:2019-03-18

摘要/Abstract

摘要： 目的研究表皮生长因子受体（EGFR）、转化生长因子-α（TGF-α）与肺癌细胞不同放疗分割剂量的关系及可能作用机制。方法对A549细胞采用常规分割（2 Gy／天）和大分割（4、8 Gy／天）照射，DT=8 Gy，采用实时荧光定量 PCR （QPCR）、酶联免疫吸附测定（ELISA）法、Western blotting分别检测A549细胞中EGFR、TGF-α基因和蛋白表达，另采用免疫荧光法测定γ-H2AX表达。结果 4 Gy／天和 8 Gy／天分割组A549细胞中EGFR、TGF-α mRNA表达水平均显著低于2 Gy／天组（P＜0.05）；4 Gy／天和 8 Gy／天分割组A549细胞中EGFR、TGF-α 蛋白表达均显著低于2 Gy／天组（P＜0.05）。免疫荧光法结果显示，4 Gy／天和 8 Gy／天分割组A549细胞中γ-H2AX表达明显高于对照组和2 Gy／天分割组。结论大剂量分割放疗后EGFR、TGF-α 表达明显下调，增加A549细胞对放射线的敏感性，其机制可能与DNA损伤修复组蛋白γ-H2AX表达上调有关。

关键词:

肺癌')">"> 肺癌, 放射治疗, EGFR, TGF-α

Abstract:

Objective To study the relationship between epidermal growth factor receptor （EGFR）， transforming growth factor-α（TGF-α） and different fractional doses of radiotherapy in lung cancer cells and its possible mechanism. Methods Lung cancer cell line A549 cells were irradiated with conventional fractionation （2Gy／day） and large fractionation （4，8 Gy／day）， DT=8 Gy. Real-time fluorescence quantitative PCR （QPCR）， enzyme-linked immunosorbent assay （ELISA） and Western blotting were used to detect EGFR， TGF-α gene and protein expression in A549 cells， respectively. In addition， the expression of γ-H2AX was determined by immunofluorescence assay. Results The expression levels of EGFR mRNA and TGF-α mRNA in A549 cells of 4 Gy／day and 8 Gy／day fractionation groups were significantly lower than those of 2 Gy／day fractionation group （P＜0.05）. The expressions of EGFR and TGF-α proteins in A549 cells of 4 Gy／day and 8 Gy／day fractionation groups were significantly lower than those of 2 Gy／day fractionation group （P＜0.05）. Immunofluorescence assay showed that the expression ofγ-H2AX in A549 cells in 4 Gy／day and 8 Gy／day fractionation groups was significantly higher than that in control group and 2 Gy／day fractionation group. Conclusion The expression of EGFR and TGF-α decreased significantly after high dose fractionated radiotherapy， which increased the sensitivity of A549 cells to radiation. The mechanism may be related to the up-regulation of the expression of histone γ-H2AX in DNA damage repair.

Key words: Lung cancer, Radiotherapy, EGFR, TGF-α

中图分类号:

R730.55

葛琴, 钱霞, 谢国栋, 蔡晶, 杨百霞, 吴建亭, 赵季忠, 崔娟娟, 朱兴华, 储春霞. EGFR和TGF-α在肺癌细胞放疗增敏中的作用[J]. 临床肿瘤学杂志, 2019, 24(2): 145-148.

GE Qin, QIAN Xia, XIE Guodong, CAI Jing, YANG Baixia, WU Jianting, ZHAO Jizhong, CUI Juanjuan, ZHU Xinghua, CHU Chunxia. . The effect of EGFR and TGF-α in radiosensitization of lung cancer cells[J]. Chinese Clinical Oncology, 2019, 24(2): 145-148.

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