Abstract: Objective To explore the value of gene expression of P16INK4A， Brn-3a and C-myc as well as the activity of telomerase in diagnosis of cervical carcinoma. Methods Cancer tissues from 40 cases of cervical cancer undergoing surgical treatment in our hospital from 2012 March to 2014 May were collected for the detection of P16INK4A， Brn-3a， C-myc and telomerase. Meanwhile， cervical tissues from 28 cases of normal cervical patients and 40 cases of cervical intraepithelial neoplasia were chosen for comparison. The receiver operating characteristic curve （ROC） was employed to evaluate the diagnostic value of gene expression of P16INK4A， Brn-3a and C-myc as well as the activity of telomerase on cervical carcinoma. Results The positive expression rates of P16INK4A， Brn-3a and C-myc were 100.0％， 95.0％ and 100.0％ in cervical cancer， significantly higher than those in normal cervical and CIN tissues with statistical difference （P＜0.05）. P16INK4A positive expression rates of CIN II-III were higher than those of the normal cervical and CIN I tissues （P＜0.05）. The positive expression rates of Brn-3a and C-myc in normal cervical tissues were 7.1％， significantly lower than those of other groups （P＜0.05）.The level of telomerase activity in tissues from cervical cancer is （44.38±3.82） U／g， higher than other groups （P＜0.05）. Conclusion There were abnormal pattern of gene expression of P16INK4A， Brn-3a and C-myc as well as activity of telomerase， likely to be associated with the pathogenesis and development of cervical cancer. The above parameters can be taken as reference indices for the diagnose of cervical cancer.